1. 제 목 : 나노플라즈몬을 이용한 세포내 분광학 이미징과 분자 검출
Nanoplasmonic Spectroscopic Imaging for Living Cells

2. 연 사 : 최연호 교수(고려대학교 생체의공학과)

3. 일 시 : 2011년 4월 14일 (목) 17:00~

4. 장 소 : 301동 1512호

5. 내 용 :
Label-free, sensitive and selective detection methods with high spatial
resolution are critically required for future applications in chemical sensor,
biological sensor, and nanospectroscopic imaging. Here I describe the
development of Plasmon Resonance Energy Transfer (PRET)-based molecular
imaging in living cells as the first demonstration of intracellular imaging with
PRET-based nanospectroscopy. In-vivo PRET imaging relied on the overlap
between plasmon resonance frequency of gold nanoplasmonic probe (GNP)
and absorption peak frequencies of conjugated molecules, which leads to
create ‘quantized quenching dips’ in Rayleigh scattering spectrum of GNP.
The position of these dips exactly matched with the absorption peaks of
target molecules. As another innovative application of PRET, I present a
highly selective and sensitive detection of metal ions by creating conjugated
metal-ligand complexes on a single GNP. In addition to conferring high
spatial resolution due to the small size of the metal ion probes (50 nm in
diameter), this method is 100 to 1,000 folds more sensitive than organic
reporter-based methods. Moreover, this technique achieves high selectivity
due to the selective formation of Cu2+complexes and selective resonant
quenching of GNP by the conjugated complexes. Since many metal ion
ligandcomplexes generate new absorption peak due to the d-d transition in
the metal ligand complex when a specific metal ion is inserted into the
complex, we can match with the scattering frequency of nanoplasmonic
metal ligand systems and the new absorption peak

6. 문 의 : 880-1916