Department News
Biochip Development for Protein Pretreatment Using in Situ Laser-Patterning Nano
Seminar Date
2004-11-15
Author
권해선
Date
2004-11-15
Views
1901
1. 제 목 : Biochip Development for Protein Pretreatment Using in Situ Laser-Patterning Nanoporous Polymer Membranes
2. 연 사 : 송시몬 교수 (한양대학교 기계공학부)
3. 일 시 : 2004년 11월 19일 (금) 16:30
4. 장 소 :
서울대 신공학관(301동) 1512호 세미나실 (화상강의로 진행)
한양대 공업센터 4층 세미나실
5. 내 용 :
Real-world biological samples require extensive pretreatmentsuch as desalting and preconcentration before they can be analyzed in a miniaturized device.This presentation will address a novel method to integrate protein(micro)dialysis and preconcentration on microchips. We developed a unique technique to laser-pattern nanoporous polymer membranes in microchip using a projection lithography and phase separation polymerization. We can engineer the membrane size, shape and the pore size.
For microdialysis, we built two mass exchangers in fused silica microchip.Both have 35 mm thick membranes fabricated inside 5 mm long microchannel, but one has membranes with molecular weight cutoff (MWCO of 2kD, and the other with 100kD. Results indicate that the mass exchanger with 2kD MWCO membranes can be used for desalting protein solutions while the mass exchanger with 100 kD can be used to separate cytosolic proteins from cell membrane fragments based on size.
For preconcentration we cast 50 mm thick membrane at the junction of a simple cross channel. Proteins electrophoretically stack at the membrane surface upon applying electric field across the membrane. Results show that the protein concentration increases at least by two orders of magnitude and that the increase is linearly proportion to concentration time and electric field strength applied. This is the first sample preconcentrator using porous media that shows the linearity complying to the theory.
6. 약 력 :
2004. 09 한양대 기계공학부 전임강사
2002. 10 - 2004. 09 Post Doc, Sandia National Labs, CA USA
2002. 04 - 2002. 09 Research Associate, Stanford Univ. CA USA
2002. 04 Ph.D Mech. Eng. Stanford Univ., CA USA
1997. 09 MS Mech. Eng. Stanford Univ. CA USA
1995. 02 한양대 기계공학과 학사
7. 문 의 :
기계항공공학부 민경덕 교수 (☏ 880-1661)
서울대 BK21 기계분야사업단 행정실 (☏ 880-7132)
8. 기계분야 박사과정 세미나 수업 수강생 여러분들은 필히 참석해 주시고,
기타 관심 있는 분들의 많은 참여를 부탁 드립니다.